Anti-SSCA1/SerpinB3 Monoclonal Antibody for WB, IF/ICC, ELISA - P29508

Core Product Specifications and Parameters

Biological Background: Serpin B3 Function and Localization

• Serpin B3, also known as Squamous Cell Carcinoma Antigen 1 (SCCA-1) or Protein T4-A, is a member of the serpin superfamily of serine protease inhibitors.
• Encoded by the SERPINB3 gene, it functions as a papain-like cysteine protease inhibitor, potentially modulating the host immune response against tumor cells.
• Serpin B3 also acts as an inhibitor of UV-induced apoptosis by suppressing the activity of c-Jun NH(2)-terminal kinase (JNK1).
• The protein is predominantly localized in the cytoplasm.
• Tissue expression is mainly in squamous cells and has been detected in some hepatocellular carcinomas at the protein level.
• Post-translational modifications include acetylation, and the protein undergoes alternative splicing.
• Keywords from UniProt: 3D-structure, Acetylation, Alternative splicing, Cytoplasm, Direct protein sequencing, Protease inhibitor, Proteomics identification, Reference proteome, Serine protease inhibitor.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 2-390 of human Serpin B3, covering nearly the full-length protein, which may support recognition of native conformations in applications like IF/ICC.
• For Western blotting, the predicted molecular weight is approximately 45 kDa; consider using a 10% SDS-PAGE gel for optimal separation.
• In IF/ICC experiments, appropriate fixation and permeabilization protocols for cytoplasmic targets should be employed to ensure antibody accessibility.
• When testing cross-reactivity with mouse or rat samples, validate expression levels and sequence homology of the target in the relevant species.
• ELISA performance may depend on the specific plate coating and detection format; optimization of coating antigen concentration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to IVD raw materials like this anti-SSCA1/SerpinB3 monoclonal antibody. Whether you require validated antibody pairs, bulk quantities of optimized monoclonals, or ancillary reagents for serine protease inhibitor research and squamous cell carcinoma biomarker development, our one-stop sourcing platform bridges your project from concept to clinic. We also assist in sourcing rare-target raw materials to meet specialized assay needs.