Anti-SSB Rabbit Polyclonal Antibody (pAb) for WB, IHC-P, IF/ICC, IP, ELISA - P05455

Core Product Specifications and Parameters

Biological Background: SSB Function and Localization

• SSB encodes the Lupus La protein, also known as La autoantigen, La ribonucleoprotein, and Sjögren syndrome type B antigen (SS-B). It is a ubiquitously expressed nuclear phosphoprotein that belongs to the La-related protein (LARP) family.
• The primary function of SSB is to bind the 3′ poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonucleolytic degradation and facilitating their correct folding and maturation. Related references: PMID:2470590, PMID:3192525
• During Coxsackievirus B3 infection, SSB binds the viral internal ribosome entry site (IRES) and stimulates IRES-mediated translation, highlighting its role in viral replication. Related references: PMID:12384597
• SSB is predominantly localized to the nucleus, consistent with its involvement in RNA processing and metabolism.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may regulate its activity and interactions.
• SSB is a known autoantigen in systemic lupus erythematosus (SLE) and Sjögren’s syndrome, making it a clinically relevant biomarker for autoimmune diagnostics.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 166–408 of human SSB, which includes a portion of the RNA-binding domain; thus, the antibody may recognize epitopes relevant to both free and RNA-bound forms of the protein.
• For Western blotting (WB), a band around 47 kDa is expected in human cell lysates; consider using nuclear extracts to enrich the signal.
• In immunohistochemistry (IHC) and immunofluorescence (IF), permeabilization and antigen retrieval steps are essential to access the nuclear target; validate staining with relevant tissue controls.
• For immunoprecipitation (IP), confirm that the antibody can capture SSB-RNA complexes; inclusion of RNase inhibitors or mild RNase treatment may influence interaction partners.
• ELISA-based detection may require optimization of coating conditions and pairing with appropriate secondary antibodies; test cross-reactivity if using denatured vs. native antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories reliable access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For researchers studying SSB in autoimmunity or RNA biology, we provide rapid sourcing of this rabbit anti-SSB polyclonal antibody alongside customized technical support to accelerate assay development. Whether you need rare target reagents or scalable supply, CamelBio simplifies procurement from concept to clinic.