Anti-SRSF7 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16629

Core Product Specifications and Parameters

Biological Background: SRSF7 Function and Localization

• SRSF7, also known as 9G8 or SFRS7, is a serine/arginine-rich (SR) splicing factor that belongs to the SR protein family. It is involved in both constitutive and alternative pre-mRNA splicing.
• It functions as a core component of the spliceosome, participating in the recognition and selection of splice sites during pre-mRNA processing.
• SRSF7 also acts as a splicing repressor; for example, it represses the splicing of MAPT/Tau exon 10, influencing tau protein isoform expression.
• Beyond splicing, SRSF7 serves as an mRNA export adapter, mediating the nuclear export of specific mRNAs such as histone H2A via interaction with the NXF1-NXT1 heterodimer (TAP/NXF1 pathway).
• The protein exhibits semi-sequence specific RNA-binding activity and enhances the RNA-binding capacity of the NXF1-NXT1 complex.
• Subcellularly, SRSF7 localizes to both the nucleus and the cytoplasm, consistent with its dual roles in splicing and mRNA export.
• According to UniProt, SRSF7 is subject to post-translational modifications including phosphorylation, acetylation, and ubiquitin-like conjugation, which may regulate its activity.
• It contains a zinc-finger domain and is expressed in a variety of tissues, including brain, liver, kidney, and lung.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 60–120 of human SRSF7, a region that may be conserved across species; consider verifying sequence homology for your target organism.
• For Western blot (WB), SRSF7 has a predicted molecular weight of ~27 kDa; ensure appropriate gel percentage and transfer conditions to detect this band.
• When performing immunohistochemistry (IHC-P), antigen retrieval protocols may influence staining; optimization for your specific tissue type is recommended.
• In immunofluorescence (IF/ICC), SRSF7 may localize to both nuclear and cytoplasmic compartments; include appropriate permeabilization and controls to distinguish subcellular distribution.
• For ELISA applications, titrate the antibody concentration against the recombinant protein or cell lysates to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

SRSF7 is a key player in pre-mRNA splicing and mRNA export, making it a valuable target for RNA processing research and diagnostic development. CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies like anti-SRSF7, bulk ancillary reagents, and sourcing for rare targets across the splicing pathway. Whether you are developing routine assays or exploring novel biomarkers, our one-stop supply platform bridges the gap from concept to clinic with reliable, research-grade materials and expert technical consultation.