Anti-SRSF6 (SRp55) Rabbit Polyclonal Antibody for WB and ELISA - Q13247

Core Product Specifications and Parameters

Biological Background: SRSF6 Function and Localization

• SRSF6, also known as SFRS6 or SRp55, is a serine/arginine-rich splicing factor that plays a key role in constitutive pre-mRNA splicing and alternative splice site selection.
• It modulates alternative splicing of MAPT/Tau exon 10, influencing tau protein isoform expression.
• SRSF6 binds to alternative exons of TNC (tenascin-C) pre-mRNA and promotes expression of alternatively spliced TNC isoforms.
• The protein contributes to wound healing and regulates keratinocyte differentiation and proliferation through alternative splicing mechanisms.
• Subcellularly, SRSF6 localizes to the nucleus and accumulates in nuclear speckles, dynamic structures enriched in splicing factors.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, and the protein contains RNA-binding motifs and RS domains.
• Molecular weight is approximately 39.6 kDa (UniProt), though the product detects a band at 40 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the N-terminal region (aa 1–100) of human SRSF6. This should detect all isoforms that contain this region, but isoform-specific detection should be verified in your system.
• For Western blotting, expect a band around 40 kDa. Optimize lysate preparation and antibody dilution for your cell line or tissue type; consider using a positive control such as HeLa or HEK293 whole cell lysate.
• This antibody is suitable for ELISA; consider pairing with a matched detection reagent for sandwich ELISA applications.
• Cross-reactivity is reported for human, mouse, and rat, making it suitable for rodent models; validate in the specific species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With this anti-SRSF6 antibody, research and diagnostic developers gain reliable access to a critical splicing factor target, supported by our comprehensive supply chain and technical consulting services.