Anti-SRSF4 Polyclonal Antibody for WB, ELISA - Q08170

Core Product Specifications and Parameters

Biological Background: SRSF4 Function and Localization

• SRSF4, also designated SFRS4 or SRP75, is a member of the serine/arginine-rich (SR) protein family of pre-mRNA splicing factors.
• It functions as an alternative splicing regulator, influencing splice site selection during pre-mRNA processing.
• Specifically, SRSF4 represses the inclusion of exon 10 in the MAPT/Tau transcript, modulating tau protein isoform expression.
• The protein localizes to nuclear speckles, dynamic subnuclear structures where splicing factors are concentrated.
• SRSF4 is post-translationally modified by phosphorylation, which regulates its activity and interactions within the spliceosome.
• Keywords associated with SRSF4 include mRNA processing, mRNA splicing, phosphoprotein, and RNA-binding, highlighting its essential role in gene expression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-180 of human SRSF4, covering the N-terminal region. Consider using this region as a reference for epitope mapping.
• This antibody is suitable for western blot (WB) and ELISA applications. For WB, a band around 57 kDa is expected based on the predicted molecular weight.
• The antibody cross-reacts with mouse and rat SRSF4, enabling multi-species studies. Validate in the relevant sample system with appropriate controls.
• Nuclear extracts may enhance detection due to SRSF4’s localization in nuclear speckles. Optimize sample preparation and antibody dilution for your specific assay conditions.
• Ensure storage at recommended temperatures and avoid repeated freeze-thaw cycles to maintain antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, covering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For splicing research targets like SRSF4, we facilitate access to high-quality primary antibodies and support materials, streamlining your diagnostic development from concept to clinic.