Anti-SRSF3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P84103

Core Product Specifications and Parameters

Biological Background: SRSF3 Function and Localization

• SRSF3 (also known as SFRS3, SRp20) is a member of the serine/arginine-rich (SR) family of splicing factors.
• It functions as a splicing factor by binding the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' in pre-mRNA, promoting specific exon inclusion during alternative splicing. Related references: PMID:17036044 PMID:26876937 PMID:32440474
• Interaction with the m6A reader YTHDC1 facilitates recruitment of SRSF3 to its mRNA-binding elements near m6A sites within exons. Related references: PMID:26876937
• Acts as an adaptor for mRNA nuclear export by binding mRNA and transferring it to the NXF1-NXT1 heterodimer (TAP/NXF1 pathway), enhancing NXF1-NXT1 RNA-binding activity. Related references: PMID:11336712 PMID:18364396
• Mediates nuclear export of m6A-containing mRNAs through interaction with YTHDC1, coordinating binding to both SRSF3 and NXF1. Related references: PMID:28984244
• Subcellular localization includes the nucleus, nuclear speckles, and cytoplasm.
• Post-translational modifications include phosphorylation and acetylation, which are critical for its regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-90 amino acids. For blocking experiments, consider using full-length recombinant SRSF3 or an N-terminal peptide.
• For Western blot, a specific band at approximately 19 kDa is expected. Validate in lysates from human, mouse, or rat cells/tissues.
• Optimal working dilutions for IHC-P, IF/ICC, and ELISA should be determined empirically; include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored procurement of rare targets. For research on RNA processing and splicing, our anti-SRSF3 antibody supports your assay development with reliable performance and flexible supply options.