Anti-SRSF2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q01130

Core Product Specifications and Parameters

Biological Background: SRSF2 Function and Localization

• SRSF2, also known as SC35 or PR264, is a key serine/arginine-rich (SR) splicing factor essential for pre-mRNA processing.
• It is necessary for the formation of the earliest ATP-dependent splicing complex and bridges U1 snRNP (5’ splice site) and U2AF (3’ splice site) during spliceosome assembly.
• The protein binds to purine-rich RNA sequences, such as 5’-AGSAGAGTA-3’ and 5’-GTTCGAGTA-3’, and commits specific transcripts like beta-globin mRNA to the splicing pathway.
• SRSF2 is localized in the nucleus, predominantly in nuclear speckles and the nucleoplasm, consistent with its role in mRNA processing.
• Phosphorylation by SRPK2 regulates its splicing activity and is required for cisplatin-induced apoptosis; acetylation is also associated with its regulation.
• As a member of the SR protein family, SRSF2 is subject to alternative splicing itself, producing multiple isoforms (e.g., SFRS2A, SRp30b).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the N-terminal region (aa 1-100), which may detect various SRSF2 isoforms; confirm specificity by siRNA knockdown or using isoform-specific controls.
• For Western blotting, a band around 25 kDa is expected; validate in the relevant sample system and under reducing conditions.
• For IHC-P, consider antigen retrieval methods and validate in formalin-fixed paraffin-embedded tissue sections from human, mouse, or rat.
• Cross-reactivity with mouse and rat has been reported; sequence alignment of the immunogen region across species may support this, but independent validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined supply chain for IVD raw materials, covering mRNA splicing and RNA-binding protein targets like SRSF2. Our network delivers validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, supporting your assay development from concept to clinic. Whether you need this anti-SRSF2 polyclonal antibody or other rare-target splicing factor raw materials, CamelBio serves as your dedicated sourcing bridge.