Anti-SRSF1/SF2/ASF Rabbit Polyclonal Antibody for WB and ELISA - Q07955

Core Product Specifications and Parameters

Biological Background: SRSF1 Function and Localization

• SRSF1, also known as ASF/SF2, is a prototypical serine/arginine-rich (SR) protein that belongs to the SR protein family of splicing factors.
• It plays a critical role in pre-mRNA splicing by preventing exon skipping and ensuring splicing accuracy; it bridges U1 snRNP and U2AF via its RS domain.
• SRSF1 binds purine-rich RNA sequences, such as the octamer 5'-RGAAGAAC-3' or decamers AGGACAGAGC/AGGACGAAGC, and three octamer copies form a powerful splicing enhancer (ASE).
• Alternative splicing generates isoforms ASF-2 and ASF-3, which function as splicing repressors rather than activators.
• In addition to splicing regulation, SRSF1 may act as an mRNA export adapter, facilitating nuclear export through the TAP/NXF1 pathway.
• Subcellular localization includes nuclear speckles (primary), cytoplasm, and chromatin, consistent with its dynamic roles in RNA processing and transport.
• The protein undergoes multiple post-translational modifications, including phosphorylation, methylation, and acetylation, which modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (a.a. 1-248) of human SRSF1, so the antibody may recognize all known isoforms; however, isoform-specific reactivity should be validated.
• For Western blot, the predicted molecular weight is approximately 28 kDa, but phosphorylated forms may migrate slightly slower; consider using phosphatase inhibitors in lysis buffers.
• For ELISA, the antibody may be used to detect purified recombinant SRSF1 or endogenous protein in cell lysates; optimization of coating and detection conditions is recommended.
• Cross-reactivity with mouse and rat orthologs is indicated; confirm performance in your specific sample system before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. With expertise in splicing factor research, our supply chain supports the development of robust assays for alternative splicing and mRNA processing targets. Contact us to discuss how we can meet your raw material needs efficiently.