Anti-SRSF1/SF2/ASF Monoclonal Antibody for WB, IHC-P, ELISA - Q07955

Core Product Specifications and Parameters

Biological Background: SRSF1 Function and Localization

• SRSF1 (Serine/arginine-rich splicing factor 1), also known as ASF-1, SF2, or SFRS1, is a member of the SR protein family that plays essential roles in constitutive and alternative pre-mRNA splicing. Two shorter isoforms, ASF-2 and ASF-3, act as splicing repressors.
• It prevents exon skipping and ensures splicing accuracy by forming a bridge between U1 snRNP and U2AF, promoting splice site recognition. SRSF1 stimulates U1 snRNP binding to 5’ splice sites and interacts with other spliceosomal components via its RS domain.
• SRSF1 binds purine-rich RNA sequences, including the octamer 5’-RGAAGAAC-3’ and the decamers AGGACAGAGC/AGGACGAAGC. It preferentially binds the 5’-CGAGGCG-3’ motif in vitro. Three copies of the octamer constitute the ASF/SF2 splicing enhancer (ASE), which specifically activates ASE-dependent splicing.
• Beyond splicing, SRSF1 may function as an mRNA export adapter, facilitating nuclear export via the TAP/NXF1 pathway.
• Subcellularly, SRSF1 localizes to nuclear speckles, the cytoplasm, and chromosomes, reflecting its dynamic roles in RNA processing and transport.
• The protein is subject to extensive post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, which regulate its activity and localization.
• Genetic variants in SRSF1 have been associated with intellectual disability, highlighting its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of SRSF1 is approximately 28 kDa, but post-translational modifications may cause band shifts or multiple bands. Validate detection in relevant cell or tissue lysates.
• In immunohistochemistry (IHC-P), optimization of antigen retrieval and blocking steps is recommended. A nuclear speckled staining pattern may be expected; validate with appropriate positive and negative controls.
• For ELISA, the antibody may be used as a capture or detection reagent; note that it was raised against a peptide immunogen (aa 100-200), so it likely recognizes a linear epitope. Perform cross-reactivity and matrix effect assessments with relevant sample types.
• Cross-reactivity with mouse and rat SRSF1 allows for multispecies studies, but always confirm specificity in the experimental system.
• As a monoclonal antibody, lot-to-lot consistency is expected, but each new lot should be verified in the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner from concept to clinic, offering one-stop access to premium IVD raw materials, technical support, and consulting tailored to diagnostic developers and researchers. This anti-SRSF1 monoclonal antibody supports the study of splicing regulation and its implications in cancer and neurodegenerative diseases. Contact us to source validated antibody pairs, bulk ancillary reagents, or custom antibodies for challenging targets, empowering your assay development and scale-up.