Anti-SRRT Rabbit Polyclonal Antibody for IHC-P, ELISA - Q9BXP5

Core Product Specifications and Parameters

Biological Background: SRRT Function and Localization

• SRRT (Serrate RNA effector molecule homolog), also known as ARS2 (arsenite-resistance protein 2), is encoded by the SRRT gene in humans. It is a ubiquitously expressed protein with a molecular weight of approximately 101 kDa.
• Acts as a mediator between the cap-binding complex (CBC) and the primary microRNA (miRNA) processing machinery, contributing to the stability and delivery of capped primary miRNA transcripts for processing by DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing.
• Involved in cell cycle progression at S phase and modulates arsenic sensitivity, although it does not directly confer arsenite resistance.
• Independently of its miRNA activity, SRRT is necessary and sufficient to promote neural stem cell self-renewal by directly binding the SOX2 promoter and positively regulating its transcription (By similarity).
• Subcellular localization is primarily in the nucleus (nucleoplasm) and cytoplasm.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination (isopeptide bond), as indicated by keyword annotations. Additional keywords: activator, alternative splicing, transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 623-872) of human SRRT, ensuring specific recognition of the full-length protein and potential isoforms.
• For IHC-P, consider using formalin-fixed paraffin-embedded sections with heat-induced antigen retrieval in citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) for optimal epitope exposure.
• In ELISA applications, this polyclonal antibody can be used for both capture and detection, but pairwise testing with monoclonal antibodies is recommended for sandwich ELISA format.
• Validate the antibody’s performance in the relevant sample system, as cross-reactivity has been confirmed for human, mouse, and rat; however, tissue-specific expression patterns may require optimization of concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For miRNA processing and transcription regulation research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-SRRT pAb, and bulk ancillary reagents. Whether you need rare target raw-material sourcing or comprehensive support from concept to clinic, CamelBio bridges the gap with reliable supply solutions.