Anti-SRPX Polyclonal Antibody for WB, IHC-P, ELISA - P78539

Core Product Specifications and Parameters

Biological Background: SRPX Function and Localization

• SRPX, encoded by the SRPX (ETX1) gene, is a sushi repeat-containing protein that belongs to the complement control protein (CCP) module family, implicated in cell adhesion and signal transduction.
• The protein undergoes alternative splicing, producing multiple isoforms.
• SRPX is anchored at the cell surface.
• It may function in phagocytosis during retinal disk shedding, cell adhesion to non‑pigment epithelial cells, or intracellular signaling.
• At the protein level, SRPX is detected in fibroblasts. Related references: PMID:36213313
• Expression is prominent in retina and heart; lower levels are found in placenta, pancreas, lung, liver, skeletal muscle, kidney, and brain.
• The protein is classified as a glycoprotein and proteoglycan, featuring disulfide‑bonded sushi domains.

Experimental Guidance and Technical Tips

• The immunogen covers residues 40‑320 of human SRPX, which includes the extracellular sushi domains; this design should recognize all major isoforms. Validate specificity in your system using positive and negative controls.
• For Western blot, a band near 52 kDa is expected under reducing conditions. Due to glycosylation and potential proteoglycan modifications, the observed molecular weight may be higher; consider deglycosylation treatments if precise size confirmation is needed.
• In IHC‑P, strong staining is anticipated in retina and heart; include tissue sections from positive (retina/heart) and negative (e.g., liver) organs as internal controls. Optimize antigen retrieval and antibody dilution empirically.
• This antibody is suitable for ELISA. Cover a range of concentrations when testing against recombinant protein or native samples to establish a reliable standard curve.
• Cross‑reactivity with mouse is reported; if working with mouse tissues, confirm consistency of reactivity across sample types and background levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with consolidated access to high‑quality IVD raw materials. This anti‑SRPX polyclonal antibody supports investigations into phagocytosis, cell adhesion, and retinal biology, and we can additionally supply validated antibody pairs, optimized monoclonal reagents, ancillary bulk reagents, and custom sourcing for rare targets—streamlining your development from concept to clinic.