Anti-SRPR Rabbit Polyclonal Antibody for WB, ELISA - P08240

Core Product Specifications and Parameters

Biological Background: SRPR Function and Localization

• SRPR is a component of the signal recognition particle (SRP) complex receptor (SR). Related references: PMID:16439358
• In conjunction with the SRP complex, SRPR ensures the correct targeting of nascent secretory proteins to the endoplasmic reticulum membrane system. Related references: PMID:16675701
• SRPR forms a guanosine 5'-triphosphate (GTP)-dependent complex with the SRP subunit SRP54; receptor compaction and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER. Related references: PMID:34020957
• The protein is encoded by the SRPRA (SRPR) gene in humans, and alternative splicing generates different isoforms.
• Subcellular localization studies indicate that SRPR is an integral membrane protein of the endoplasmic reticulum.
• Post-translational modifications include phosphorylation; the protein contains GTP-binding and nucleotide-binding domains, consistent with its role as a molecular switch.
• Keywords associated with SRPR include 3D-structure, alternative splicing, endoplasmic reticulum, GTP-binding, membrane, nucleotide-binding, phosphoprotein, proteomics identification, receptor, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-220 of human SRPR (NP_003130.2), covering the N-terminal region. This region may detect both full-length protein and potential isoforms; validation in the relevant sample system is recommended.
• For Western blot (WB), the predicted molecular weight is approximately 70 kDa. Optimize antibody dilution and use appropriate positive controls, such as human cell lines expressing SRPR.
• The antibody is also supplied for ELISA; consider testing antigen coating conditions and detection ranges experimentally.
• As a polyclonal antibody, lot-to-lot variability is possible; include internal controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic and research workflows. For studies on protein translocation and ER targeting, this anti-SRPR polyclonal antibody is part of our extensive catalog, complementing our capability to source rare-target raw materials and offer technical consulting from concept to clinic.