Anti-SRPK2 Rabbit Polyclonal Antibody for WB and ELISA - P78362

Core Product Specifications and Parameters

Biological Background: SRPK2 Function and Localization

• SRSF protein kinase 2 (SRPK2) is a serine/arginine-rich protein-specific kinase that phosphorylates substrates at serine residues within RS domains, thereby regulating pre‑mRNA splicing. Related references: PMID:18559500, PMID:21056976, PMID:9472028
• Promotes neuronal apoptosis by up‑regulating cyclin‑D1 (CCND1) expression through phosphorylation of SRSF2, which suppresses p53/TP53 phosphorylation and relieves repression on CCND1. Related references: PMID:19592491, PMID:21205200
• Essential for spliceosomal B complex formation via phosphorylation of DDX23/PRP28. Related references: PMID:18425142
• Contributes to genome stability by suppressing incorrect R‑loops during transcription, likely through DDX23 phosphorylation. Related references: PMID:28076779
• Phosphorylates ACIN1, causing its redistribution from nuclear speckles to the nucleoplasm and leading to cyclin A1 (but not cyclin A2) up‑regulation. Related references: PMID:18559500
• Mediates hepatitis B virus (HBV) core protein phosphorylation and negatively regulates HBV replication by reducing pregenomic RNA packaging efficiency. Related references: PMID:12134018, PMID:16122776
• Localizes to the cytoplasm, nucleus, nucleoplasm, nuclear speckles, and chromosome.
• Highly expressed in brain, moderately in heart and skeletal muscle, and at low levels in lung, liver, and kidney.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 420–520 within the C‑terminal region of human SRPK2; consider this when designing complementary detection reagents.
• For Western blot, a band of approximately 78 kDa is expected; validate using whole‑cell lysates from brain or heart tissues where SRPK2 is abundant.
• This antibody is reactive with human, mouse, and rat samples; cross‑species consistency should be confirmed in your specific experimental system.
• For ELISA applications, consider optimizing coating and detection conditions with the relevant recombinant protein or endogenous analyte.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting manufacturers and researchers in splicing and kinase biology. Alongside this SRPK2 polyclonal antibody, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. For rare or custom targets, our network bridges the gap from concept to clinic.