Anti-SRPK1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96SB4

Core Product Specifications and Parameters

Biological Background: SRPK1 Function and Localization

• SRPK1 is an ATP-dependent serine/threonine protein kinase that specifically phosphorylates SR splicing factors at serine residues within RS domains, regulating pre-mRNA splicing.
• It controls the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis.
• Beyond splicing, SRPK1 influences mRNA maturation, chromatin reorganization in somatic and sperm cells, and cell cycle progression.
• Isoform 2 phosphorylates substrates including SFRS2, ZRSR2, LBR, and PRM1, mediates HBV core protein phosphorylation, and negatively regulates HBV replication by reducing pgRNA packaging efficiency.
• Isoform 1 and isoform 2 both promote splicing of exon 10 in MAPT/TAU, and their ratio dictates cell fate in K-562 leukemic cells (isoform 2 supports proliferation, isoform 1 differentiation).
• SRPK1 phosphorylates the N-terminus of ERC1. Related references: PMID:30745339
• The protein is localized to the cytoplasm, nucleus, nucleoplasm, nuclear speckles, chromosomes, and microsomes.
• Tissue-specific expression: isoform 2 is predominant in testis and also present at lower levels in several other tissues; isoform 1 is testis-specific and less abundant.
• SRPK1 is a phosphoprotein that belongs to the protein kinase superfamily and contains an ATP-binding pocket.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 290–480 of human SRPK1 (NP_003128.3). Consider verifying the sequence conservation if using in mouse or rat models.
• For Western blot, the target protein weight is approximately 74 kDa. Include appropriate loading controls and optimize blocking conditions.
• Immunofluorescence/ICC experiments should account for SRPK1’s nuclear and cytoplasmic localization; standard fixation and permeabilization may be suitable.
• ELISA protocols may benefit from peptide-coated plates or a sandwich format; pair with a matched detection antibody if needed.
• Validate antibody performance in the relevant sample system, as cross-reactivity has been demonstrated with human, mouse, and rat.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for splicing regulation and kinase research. Need a specific clone or rare target material? Our procurement network and technical guidance streamline your project from R&D to production.