Anti-SRPK1 Monoclonal Antibody for WB, IHC-P, ELISA - Q96SB4

Core Product Specifications and Parameters

Biological Background: SRPK1 Function and Localization

• SRPK1 (SFRS protein kinase 1) is a serine/arginine-rich protein-specific kinase from the serine/threonine kinase family.
• It specifically phosphorylates SR splicing factors on serine residues within RS domains, regulating pre-mRNA splicing and intranuclear distribution of splicing factors.
• Beyond splicing, SRPK1 influences mRNA maturation, chromatin reorganization in somatic and sperm cells, and cell cycle progression.
• Isoform 2 employs a directional, dual-track phosphorylation mechanism on SRSF1, involving processive and distributive steps mediated by a docking groove.
• SRPK1 is present in multiple subcellular locations: cytoplasm, nucleus, nucleoplasm, nuclear speckles, and chromosomes.
• Two isoforms exist: isoform 1 (mainly testis-specific) and isoform 2 (testis and broadly in tissues like heart, ovary, small intestine, liver, kidney, pancreas, skeletal muscle).
• SRPK1 isoform 2 mediates HBV core protein phosphorylation and negatively regulates HBV replication by reducing pgRNA packaging, independent of core phosphorylation.
• SRPK1 phosphorylates the N-terminus of ERC1 (PubMed:30745339). Related references: PMID:30745339

Experimental Guidance and Technical Tips

• SRPK1 has multiple isoforms with predicted molecular weights of 74, 72, and 92 kDa; in Western blot, expect multiple bands depending on the isoform expression in your sample.
• For IHC-P, consider antigen retrieval methods and validate using testis or other SRPK1-expressing tissues as positive controls.
• The immunogen is a synthetic peptide from the C-terminal region (aa 291-390); the antibody may recognize both isoforms but cross-reactivity with related kinases should be verified.
• Validate the antibody's performance in your specific assay system, as experimental conditions can vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-SRPK1 monoclonal antibody supports research in pre-mRNA splicing, cell cycle regulation, and host-virus interactions. Whether you need validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, or rare target raw materials, CamelBio bridges your projects from concept to clinic.