Anti-SRP9 Polyclonal Antibody for WB, IF/ICC, ELISA - P49458

Core Product Specifications and Parameters

Biological Background: SRP9 Function and Localization

• SRP9 (Signal recognition particle 9 kDa protein, also known as ALURBP) is a component of the signal recognition particle (SRP) complex.
• The SRP complex mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum.
• Together with SRP14 and the Alu portion of SRP RNA, SRP9 forms the elongation arrest domain that pauses translation until proper targeting is achieved. Related references: PMID:11089964
• The heterodimeric complex of SRP9 and SRP14 is required for SRP RNA binding.
• Subcellular localization is predominantly cytoplasmic.
• Keywords include acetylation, alternative splicing, and ribonucleoprotein, indicating post-translational modifications and the possibility of multiple isoforms.

Experimental Guidance and Technical Tips

• For western blot (WB) analysis, a band of approximately 10 kDa is expected for SRP9. Consider using a low molecular weight marker and optimize transfer conditions for small proteins.
• In immunofluorescence (IF/ICC) assays, an exclusively cytoplasmic staining pattern should be observed. Validate in relevant cell lines and include appropriate controls.
• For ELISA applications, this polyclonal antibody may be used for capture or detection; consider pairing with a monoclonal antibody to enhance specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, providing validated antibodies and reagents for signal recognition particle research. From optimized polyclonal antibodies to bulk ancillary reagents and rare target sourcing, we support your diagnostic development from concept to clinic.