Anti-SRP72 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O76094

Core Product Specifications and Parameters

Biological Background: SRP72 Function and Localization

• SRP72 is a core component of the signal recognition particle (SRP), a ribonucleoprotein complex that directs the co-translational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).
  Related references:
  PMID:34020957

• SRP72 binds the 7SL RNA, the SRP's RNA component, with low affinity alone but with significantly increased affinity in complex with SRP68, ensuring proper SRP assembly and function.
  Related references:
  PMID:21073748, PMID:27899666

• Together with the SRP complex, SRP72 recognises the signal sequence of nascent polypeptide chains and mediates their delivery to the SRP receptor (SR) at the ER membrane, facilitating GTP-dependent protein translocation.
  Related references:
  PMID:34020957

• SRP72 may contribute to the elongation arrest function of the SRP, transiently pausing translation to ensure accurate targeting to the ER (By similarity).

• Subcellularly, SRP72 is predominantly cytoplasmic and localises to the endoplasmic reticulum, aligning with its role in nascent protein trafficking.

• SRP72 is subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination, which may regulate its activity and interactions.

• Alternative splicing and missense variants in SRP72 have been linked to bone marrow failure syndromes (BMFF/BMFS1), highlighting its critical cellular function.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 440–544) maps to a conserved C-terminal region; when using rodent samples (mouse/rat), verify a single band at ~75 kDa in WB and include a positive control.
• For IHC-P and IF/ICC, consider testing a range of dilutions and use appropriate antigen retrieval methods. The cytoplasmic/ER localisation of SRP72 should guide your imaging controls.
• In ELISA, pair this recombinant rabbit monoclonal antibody with a suitable detection system and validate using recombinant SRP72 or cell lysates expressing the target.
• As a recombinant monoclonal antibody, it offers high batch consistency; however, always optimise conditions for your specific sample type and assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides complete raw-material solutions for diagnostic manufacturers and research institutes, from early-stage R&D to clinical applications. This high-quality rabbit monoclonal anti-SRP72 antibody supports investigations into protein translocation, ER biology, and related disorders. We facilitate your projects with validated antibody pairs, custom monoclonal/polyclonal antibodies, bulk recombinant proteins, and rare target sourcing—helping you move from concept to clinic with confidence.