Anti-SRP72 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O76094

Core Product Specifications and Parameters

Biological Background: SRP72 Function and Localization

• SRP72 is a subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). Related references: PMID:34020957
• The SRP complex binds signal sequences of nascent polypeptides, directing the ribosome-nascent chain complex to the SRP receptor on the ER membrane, where subsequent compaction and GTPase rearrangement facilitate protein translocation. Related references: PMID:34020957
• SRP72 binds to the SRP RNA (7SL RNA) with low affinity alone, but this interaction is significantly enhanced in the presence of SRP68. Related references: PMID:21073748, PMID:27899666
• The SRP complex is also thought to participate in elongation arrest, pausing translation until the ribosome-nascent chain complex reaches the ER membrane (By similarity).
• SRP72 is localized to both the cytoplasm and the endoplasmic reticulum, consistent with its role in protein targeting.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, as indicated by keywords and proteomics identification.
• Alternative splicing of the SRP72 gene results in multiple isoforms, and mutations in this gene are associated with disease variants (bone marrow failure syndromes).
• The protein is classified as a TPR repeat-containing protein and a ribonucleoprotein.

Experimental Guidance and Technical Tips

• This antibody was generated using a recombinant immunogen corresponding to amino acids 440-544 of human SRP72, which may be suitable for detecting full-length SRP72 in applications such as WB, IHC-P, IF/ICC, and ELISA.
• For Western blotting, expect a band around 75 kDa; validate specificity using appropriate controls, including lysates from human, mouse, and rat tissues.
• As a polyclonal antibody, consider lot-to-lot consistency and validate performance in your specific experimental setup.
• For IHC and IF, optimize antigen retrieval and dilution factors, and include isotype controls.
• The antibody has not been tested for other applications (e.g., IP, ChIP); users should pilot-test as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing high-quality antibodies like this anti-SRP72 polyclonal antibody to support research into protein translocation and ER targeting. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or assistance sourcing rare targets, CamelBio's supply network bridges the gap from concept to clinic. Contact us to explore how our comprehensive portfolio can accelerate your diagnostic development.