Anti-SRP54 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P61011

Core Product Specifications and Parameters

Biological Background: SRP54 Function and Localization

• SRP54 is the 54 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). Related references: PMID:34020957
• It recognizes and binds signal sequences of presecretory proteins emerging from ribosomes, then interacts with the SRP receptor subunit SRPRA to direct them to the ER membrane. Related references: PMID:34020957
• SRP54 exhibits basal GTPase activity and stimulates reciprocal GTPase activation of SRPRA, driving conformational rearrangements essential for protein translocation. Related references: PMID:28972538 PMID:34020957
• Stable interaction of SRP54 with the SRP RNA requires the additional subunits SRP9/SRP14 and/or SRP19.
• SRP54 localizes to the cytoplasm, endoplasmic reticulum, and nuclear speckles.
• It plays a role in proliferation and differentiation of granulocytic cells, neutrophil migration capacity, and exocrine pancreas development. Related references: PMID:28972538 PMID:29914977
• Keywords associated with SRP54 include GTP-binding, nucleotide-binding, RNA-binding, ribonucleoprotein, and alternative splicing.

Experimental Guidance and Technical Tips

• For Western blot, use a reduced sample buffer and boiling protocol; a band at approximately 56 kDa is expected. Validate loading with appropriate controls.
• For immunofluorescence, permeabilization may be required to detect intracellular SRP54; colocalization with ER markers (e.g., calnexin) can help confirm specificity.
• The immunogen corresponds to a C‑terminal sequence (amino acids 400–504); if alternative splicing removes this epitope, some isoforms may not be detected.
• Cross-reactivity with mouse and rat samples is indicated—confirm with positive and negative controls in your model system.
• For ELISA, consider optimizing coating and detection conditions using recombinant SRP54 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and life science researchers a one-stop platform for IVD raw-material sourcing, including validated monoclonal antibodies like this anti-SRP54 clone. To support SRP research and assay development, we can supply complementary raw materials such as antigen standards, matched detection antibodies, and bulk ancillary reagents. Our team streamlines procurement and can assist with sourcing rare-target reagents, bridging your project from concept to clinical application.