Anti-SRP54 Polyclonal Antibody for WB, ELISA - P61011

Core Product Specifications and Parameters

Biological Background: SRP54 Function and Localization

• Encodes the 54 kDa subunit of the signal recognition particle (SRP), a universally conserved ribonucleoprotein complex that directs nascent secretory and membrane proteins to the endoplasmic reticulum. Related references: PMID:34020957
• Recognizes and binds the hydrophobic signal sequence of presecretory proteins as they emerge from the ribosome, initiating cotranslational targeting. Related references: PMID:34020957
• Exhibits basal GTPase activity and drives reciprocal GTPase activation of the SR receptor subunit SRPRA, forming a GTP-dependent complex essential for translocation. Related references: PMID:28972538 PMID:34020957
• SRP54 function in GTP-dependent SR compaction and rearrangement is critical for efficient protein translocation across the ER membrane. Related references: PMID:34020957
• Requires the SRP9/SRP14 heterodimer and/or SRP19 for stable association with SRP RNA, ensuring proper complex assembly and function.
• Localizes to the cytoplasm, endoplasmic reticulum, and nucleus speckles, consistent with its roles in targeting, translocation, and potential nuclear functions.
• Plays a role in granulocytic cell proliferation and differentiation, neutrophil migration, and exocrine pancreas development, as evidenced by functional studies. Related references: PMID:28972538 PMID:29914977
• Annotated with keywords including alternative splicing, disease variant, and nucleotide-binding, indicating potential pathological relevance and regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 196–455 of human SRP54 (isoform 1, NP_001139754.1), covering the GTPase and M domains; verify sequence homology in your target species before use.
• For Western blot, consider a dilution range of 1:500–1:2000 and validate using a positive control such as HeLa or HEK293T whole-cell lysate, which are known to express SRP54.
• For ELISA, optimize coating antigen and antibody concentration; recombinant SRP54 protein or peptide may serve as a suitable capture reagent.
• Observed band may appear near 56 kDa, but post-translational modifications or alternative splicing could shift mobility—confirm with appropriate controls.
• As a polyclonal antibody, lot-to-lot variability may occur; include reference standards and run preliminary tests when switching lots.
• Cross-reacts with mouse SRP54, but homology with rat should be checked (98% identity) if intended for that species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. For researchers exploring SRP-dependent protein translocation and ER targeting, this anti-SRP54 polyclonal antibody provides a reliable tool to accelerate assay development. As your trusted sourcing partner, CamelBio also supports rare-target material sourcing and technical consulting to help you advance from concept to clinic seamlessly.