Anti-SRP19 Polyclonal Antibody for WB, IHC-P, ELISA - P09132

Core Product Specifications and Parameters

Biological Background: SRP19 Function and Localization

• SRP19 is a 16 kDa protein that serves as an essential component of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum.
• It directly binds to the 7SL RNA scaffold of the SRP and is required for the stable incorporation and assembly of the SRP54 protein into the SRP complex.
• The SRP19 gene is subject to alternative splicing, which may generate multiple protein isoforms with potentially distinct functions or localizations (keyword: Alternative splicing).
• Subcellularly, SRP19 is predominantly found in the cytoplasm, where the SRP cycle occurs, but it has also been detected in the nucleus, specifically within the nucleolus and nucleoplasm, suggesting possible non-canonical roles.
• Structural studies have resolved the three-dimensional conformation of SRP19, and it has been characterized as an RNA-binding ribonucleoprotein (keywords: 3D-structure, Ribonucleoprotein, RNA-binding).
• The protein has been directly sequenced and identified in proteomics screens, confirming its endogenous expression in human cells (keywords: Direct protein sequencing, Proteomics identification).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering full-length SRP19 (amino acids 1-144); the resulting polyclonal antibody may recognize linear and conformational epitopes. Consider validating in both denaturing and native conditions.
• For Western blot, the expected band is approximately 16 kDa. Pre-stain the membrane to confirm efficient transfer of low-molecular-weight proteins, and include a positive control such as HeLa or HEK293 whole-cell lysate.
• When performing IHC-P, optimize antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) and consider testing both cytoplasmic and nuclear staining patterns.
• Given cross-reactivity with mouse and rat, this antibody is suitable for comparative studies; however, signal intensity may vary due to sequence divergence—validate in the relevant species’ sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research groups with a comprehensive portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. This SRP19 antibody is relevant for studies on protein secretion and ER targeting pathways. Beyond single reagents, we also offer bulk supply options, custom antibody development, and rare-target sourcing to streamline your assay development and production needs.