Anti-SRM Polyclonal Antibody for WB, ELISA - P19623

Core Product Specifications and Parameters

Biological Background: Spermidine Synthase (SRM) Function and Localization

• Spermidine synthase (SRM), also known as putrescine aminopropyltransferase (PAPT), SPS1, SPDSY, and SRML1, is encoded by the SRM gene in humans.
• Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), a critical step in polyamine biosynthesis essential for cell growth and proliferation.
• Has a strong substrate preference for putrescine, with very low activity towards 1,3-diaminopropane and extremely low activity on spermidine.
• Classified as a transferase (EC 2.5.1.16) within the spermidine synthase family, involved in the polyamine biosynthetic pathway.
• Post-translational modifications include acetylation, and the protein is linked to disease variants; structural studies (3D-structure) and proteomics identification confirm its role as a reference proteome component.
• The predicted molecular weight is approximately 33.8 kDa, aligning with the observed 34 kDa band in Western blotting.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning the full-length human SRM (amino acids 1-302). Due to its polyclonal nature, it may recognize multiple epitopes; consider using appropriate positive and negative controls to confirm specificity.
• For Western blotting, a band around 34 kDa is expected. Optimize blocking and antibody dilution based on your sample system; validate cross-reactivity in human, mouse, and rat tissues as needed.
• In ELISA applications, titrate the antibody to determine optimal coating and detection conditions, and include well-characterized standards for quantitative analysis.
• Although validated for the indicated species, perform a pilot experiment when working with other species or sample types to verify performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated polyclonal antibodies like this SRM antibody, optimized for WB and ELISA in polyamine biosynthesis research. As a one-stop bridge, we supply bulk ancillary reagents, customized antibody pairs, and rare target sourcing services to accelerate your diagnostic development from concept to clinic. Partner with us to streamline your procurement and ensure consistent quality across your IVD workflows.