Anti-SRI Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P30626

Core Product Specifications and Parameters

Biological Background: SRI Function and Localization

• SRI encodes sorcin, a 22 kDa calcium-binding protein also referred to as CP-22 or V19.
• Sorcin plays a critical role in cardiac excitation-contraction coupling by modulating calcium homeostasis in the sarcoplasmic reticulum.
• It directly interacts with and regulates the activity of RYR2 calcium release channels on the sarcoplasmic reticulum membrane.
• Subcellularly, sorcin localizes predominantly to the cytoplasm and associates with the sarcoplasmic reticulum membrane in cardiac myocytes.
• The protein contains multiple EF-hand motifs that confer calcium-dependent conformational changes and is a member of the penta-EF-hand family.
• Alternative splicing produces distinct isoforms, and sorcin has been extensively characterized via proteomics and direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein spanning amino acids 1–198 of human SRI (NP_003121.1). Consider that this region may be suitable for detecting both full-length and truncated isoforms in Western blot under denaturing conditions.
• For IHC-P, antigen retrieval conditions may require optimization due to the dual cytoplasmic and membrane localization of SRI.
• Cross-reactivity with mouse and rat SRI is listed; researchers should validate performance in their specific sample type and species to confirm sensitivity and specificity.
• As this is a polyclonal antibody, lot-to-lot variability is possible; include appropriate positive (e.g., cardiac tissue lysate) and negative controls in each experiment.
• The antibody has been applied in ELISA; users may consider testing different coating and detection conditions for optimal signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers and research institutes, offering a seamless sourcing experience for IVD raw materials including validated antibodies against cardiac targets like SRI. Our portfolio spans monoclonal and polyclonal antibodies, bulk ancillary reagents, and technical consulting, supporting you from early-stage concept to clinical application. Whether you are developing assays for excitation-contraction coupling studies or cardiac biomarker panels, we provide flexible supply solutions and expert support to meet your needs.