Anti-SRGN Polyclonal Antibody for WB, IF-P, ELISA - P10124

Core Product Specifications and Parameters

Biological Background: SRGN Function and Localization

• SRGN (Serglycin) is a hematopoietic proteoglycan core protein, also known as platelet proteoglycan core protein or secretory granule proteoglycan core protein (UniProt: P10124).
• It plays a critical role in the formation of mast cell secretory granules and mediates the storage of various compounds, including proteases, in secretory vesicles of connective tissue and mucosal mast cells.
• SRGN is required for the storage of granzyme B in T-lymphocytes and localizes neutrophil elastase in azurophil granules of neutrophils.
• By forming a complex with granzyme B, SRGN facilitates perforin‑mediated delivery to target cells, playing a key role in cytotoxic cell granule‑mediated apoptosis.
• SRGN also mediates the processing of MMP2 and regulates the secretion of TNF, potentially influencing protease secretion and inflammatory responses.
• The protein inhibits bone mineralization, linking it to biomineralization processes.
• Subcellularly, SRGN is found in cytoplasmic granules, cytolytic granules, the Golgi apparatus, and can be secreted into the extracellular space.
• Post‑translational modifications include disulfide bonds and extensive glycosylation, characteristic of proteoglycans; it contains a signal peptide for secretion.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 59–158 of human SRGN (NP_002718.2). For WB, verify that the observed band corresponds to the mature protein mass (~18 kDa) after signal peptide cleavage and post‑translational modifications, though migration may be affected by glycosylation.
• For IF‑P, appropriate permeabilization is needed to access intracellular granular structures; validate staining in known SRGN‑expressing cells such as mast cells, T‑lymphocytes, or neutrophils.
• ELISA may be used for quantitative detection of SRGN in cell lysates or biological fluids; consider pairing with a suitable capture antibody for sandwich assays.
• Cross‑reactivity is reported with human and mouse; investigators using other species should perform independent validation.
• As a polyclonal antibody, lot‑to‑lot consistency should be confirmed in the intended application; pre‑adsorption may be considered to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw materials, offering a streamlined bridge from discovery to clinical application. This anti‑SRGN antibody supports research into secretory granule biology, apoptosis, and inflammatory disorders. Beyond high‑quality monoclonal and polyclonal antibodies, CamelBio provides validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare‑target reagents—empowering diagnostic manufacturers and laboratories with integrated supply solutions.