Anti-SRFBP1 Rabbit Polyclonal Antibody for WB, ELISA - Q8NEF9

Core Product Specifications and Parameters

Biological Background: SRFBP1 Function and Localization

• Serum response factor-binding protein 1 (SRFBP1), also known as p49/STRAP, is a transcription regulation-associated protein that may modulate SRF-dependent transcriptional activation in cardiac tissues, particularly during aging.
• It is proposed to participate in the biosynthesis and/or processing of the glucose transporter SLC2A4 in adipose cells (By similarity).
• SRFBP1 is abundantly expressed in heart and skeletal muscle, with much lower levels detected in brain and lung, indicating a muscle-enriched expression profile.
• The protein localizes predominantly to the cytoplasm, with enrichment in the perinuclear region, suggesting a role in nucleocytoplasmic transport or signaling.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, and the protein contains a coiled-coil domain, which may mediate protein-protein interactions.
• Its molecular weight is approximately 49 kDa, consistent with its predicted mass of 48.6 kDa, and it belongs to a family of proteins involved in transcriptional regulation.

Experimental Guidance and Technical Tips

• The N-terminal immunogen (residues 1-300) is conserved across species; cross-reactivity with mouse has been confirmed, making this antibody suitable for mouse cardiac or skeletal muscle studies.
• For Western blot, expect a major band near 49 kDa; heart and skeletal muscle lysates are ideal positive controls due to high endogenous expression.
• ELISA can be employed for quantitative detection; consider optimizing coating antigen and antibody dilution for best signal-to-noise ratio.
• As a polyclonal antibody, batch-to-batch consistency should be verified, and inclusion of blocking peptide or knockdown controls is recommended to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for IVD manufacturers and research labs by offering a comprehensive portfolio of raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, antigens, and ancillary reagents. This anti-SRFBP1 polyclonal antibody supports cardiac and metabolic disease research, and we can assist with custom bulk orders, rare-target sourcing, and technical consultation to accelerate your assay development pipeline.