Anti-SRF (MCM1) Rabbit Polyclonal Antibody for WB and ELISA - P11831

Core Product Specifications and Parameters

Biological Background: SRF Function and Localization

• SRF (Serum Response Factor), also designated MCM1, is a ubiquitously expressed transcription factor that binds to the serum response element (SRE) in the promoter region of immediate-early genes such as FOS.
• As a master regulator of the actin cytoskeleton, SRF forms a complex with the myocardin-related transcription factor A (MRTFA) to drive expression of cytoskeletal genes in response to Rho GTPase signaling and changes in globular actin (G-actin) levels.
• SRF is essential for cardiac development and myogenic differentiation, playing a critical role in cardiomyocyte maturation and contractile gene expression.
• The protein localizes predominantly to the nucleus and is subject to multiple post-translational modifications including phosphorylation and glycosylation, which modulate its transcriptional activity.
• SRF is classified as a developmental protein and a transcriptional activator with a conserved DNA-binding domain that recognizes the CC(A/T)6GG (CArG) box motif.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 52 kDa may be observed in nuclear extracts of human or mouse cell lines; consider using positive controls such as HeLa or NIH/3T3 cells.
• The immunogen spans the N-terminal 280 amino acids, which includes the highly conserved DNA-binding domain, supporting cross-reactivity with mouse; validate antibody performance in your specific sample system.
• For ELISA, the antibody can be employed as a capture or detection reagent; optimal dilutions and buffer conditions should be empirically determined for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive IVD raw material supply chain, delivering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. The SRF polyclonal antibody exemplifies our commitment to high-quality tools for studying cytoskeletal and cardiac gene regulation; we also offer complementary reagents to support your complete assay development from concept to clinic.