Anti-SREBP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q12772

Core Product Specifications and Parameters

Biological Background: SREBP2 Function and Localization

• SREBP2 is a precursor transcription factor embedded in the endoplasmic reticulum membrane. Under low sterol conditions, it undergoes proteolytic processing to release the N-terminal transcription factor domain that translocates to the nucleus (PMID:32322062).
  Related references: PMID:32322062

• As a key transcription factor, SREBP2 binds to sterol regulatory element 1 (SRE-1) and E-box motifs to activate genes involved in cholesterol biosynthesis, exhibiting dual DNA-binding specificity (PMID:12177166, PMID:7903453).
  Related references: PMID:12177166, PMID:7903453

• The full-length protein localizes to the endoplasmic reticulum and Golgi apparatus membranes, while the cleaved active form is transported via COPII-coated vesicles to the nucleus.

• SREBP2 is ubiquitously expressed in both adult and fetal human tissues, supporting its fundamental role in lipid metabolism.

• The protein is characterized by a basic helix-loop-helix (bHLH) domain and undergoes post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitin conjugation.

• SREBP2 plays a central role in cholesterol homeostasis and lipid metabolism, making it a critical target in metabolic disorder research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 155-310 of human SREBF2, a region that may include parts of the N-terminal regulatory domain. For WB, the predicted molecular weight is ~124 kDa; consider using appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• For IHC-P, antigen retrieval conditions should be optimized; the antibody is expected to detect both precursor (membrane) and processed (nuclear) forms.
• Cross-reactivity with mouse and rat SREBP2 has been reported; validate in your species of interest.
• As a polyclonal antibody, lot-to-lot consistency may vary; consider including appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, offering validated antibodies, antigen pairs, and ancillary reagents for diagnostic development. Our portfolio includes high-quality polyclonal antibodies like this anti-SREBP2 antibody, supporting research in cholesterol metabolism and lipid-related diseases. Whether you need stock reagents, bulk production, or customized solutions, CamelBio bridges the gap from concept to clinical application with reliable supply and technical support.