Anti-SREBF2 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q12772

Core Product Specifications and Parameters

Biological Background: SREBF2 Function and Localization

• SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2) is a basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor critical for lipid homeostasis.
• It acts as a master transcription factor for cholesterol biosynthesis and uptake, regulating genes like HMG-CoA reductase and LDL receptor.
• SREBF2 is synthesized as an inactive precursor anchored in the endoplasmic reticulum (ER) membrane. Related references: PMID:32322062
• Under low cholesterol levels, the precursor is transported to the Golgi and proteolytically processed to release the active N-terminal domain, which translocates to the nucleus to activate target gene expression. Related references: PMID:32322062
• It binds with high affinity to the sterol regulatory element 1 (SRE-1; 5′-ATCACCCCAC-3′) and also recognizes E-box motifs (5′-ATCACGTGA-3′), enabling regulation of diverse promoters. Related references: PMID:12177166 PMID:7903453
• SREBF2 is ubiquitously expressed across adult and fetal human tissues, reflecting its fundamental role in cellular cholesterol metabolism.
• The protein shuttles between the ER, Golgi, COPII vesicles, and nucleus, with subcellular distribution tightly controlled by cellular sterol status.
• Post-translational modifications including phosphorylation, ubiquitination, and isopeptide bond formation modulate its stability and activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 401–500 within the N-terminal transcription factor domain; both the full-length precursor (~124 kDa) and the cleaved nuclear form (~68 kDa) may be detected by Western blot depending on cell treatment conditions.
• For Western blots, consider using protease inhibitors and performing sterol depletion (e.g., with statins) to enrich the nuclear active form.
• In immunofluorescence experiments, permeabilization and staining of cells cultured under low-sterol conditions can enhance nuclear signal localization.
• Cross-reactivity with mouse SREBF2 is reported; however, validate the antibody in your specific mouse tissue or cell line, as expression levels can vary.
• The antibody is suitable for ELISA applications; titrate for optimal signal-to-noise ratio and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for specialist targets like SREBF2. Whether developing assays for cholesterol metabolism research or lipid disorder diagnostics, we bridge the gap from concept to clinic with reliable supply and technical consultation.