Anti-SREBF1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P36956

Core Product Specifications and Parameters

Biological Background: SREBF1 Function and Localization

• SREBF1 (also known as SREBP1, bHLHd1) encodes a sterol regulatory element-binding transcription factor that exists as a precursor embedded in the endoplasmic reticulum membrane. Upon low sterol concentrations, it is processed to release the transcription factor form that translocates to the nucleus. Related references: PMID:32322062
• Cleaved SREBF1 binds to sterol regulatory element 1 (SRE-1; 5'-ATCACCCCAC-3') and E-box motifs (5'-ATCACGTGA-3') in the promoters of genes involved in cholesterol biosynthesis and the LDL receptor pathway, thereby activating their transcription. Related references: PMID:12177166, PMID:32322062, PMID:8402897
• Alternative splicing produces isoforms with distinct activities: the SREBP-1A isoform (expressed in select tissues) has high transcriptional activity and stimulates both lipogenic and cholesterogenic gene expression, whereas the predominant SREBP-1C isoform (expressed in liver, adipose, and other tissues) primarily regulates lipogenic genes and has weaker activity. Related references: PMID:12177166, PMID:32497488
• Additionally, an isoform lacking the requirement for Golgi proteolytic processing is constitutively active in transactivating lipogenic gene promoters.
• Subcellularly, SREBF1 cycles between the endoplasmic reticulum and Golgi membranes; upon activation, the transcription factor fragment is released and translocates to the nucleus. It is also found in COPII-coated vesicles.
• SREBF1 is a phosphoprotein and is subject to ubiquitination, consistent with regulated membrane-associated transcription factors.
• The protein is widely expressed, with highest levels in liver and adrenal gland; in fetal tissues, lung and liver show peak expression. Related references: PMID:8402897
• SREBF1 plays a critical role in lipid metabolism and has been linked to disease variants such as ichthyosis.

Experimental Guidance and Technical Tips

• The recombinant immunogen (amino acids 200–310 of human SREBF1) corresponds to a conserved region, suggesting the antibody may recognize multiple isoforms and potentially cross-react with mouse and rat as listed.
• In Western blot experiments, expect a band at approximately 122 kDa corresponding to the full-length precursor; a smaller band (~68 kDa) representing the cleaved active transcription factor may also be observed depending on cellular sterol status.
• For immunofluorescence, localization may include cytoplasmic/endoplasmic reticulum patterns under sterol-replete conditions and nuclear translocation upon sterol depletion; co-staining with organelle markers is recommended.
• Optimal dilutions for WB, IF/ICC, and ELISA should be empirically determined using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams end-to-end access to IVD raw materials and technical support, from concept to clinic. For lipid metabolism and cholesterol research, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to accelerate your assay development and regulatory approval.