Anti-SRD5A2 Monoclonal Antibody for WB, ELISA - P31213

Core Product Specifications and Parameters

Biological Background: SRD5A2 Function and Localization

• SRD5A2 (3-oxo-5-alpha-steroid 4-dehydrogenase 2), also known as 5 alpha-SR2 or Type II 5-alpha reductase, is an NADPH-dependent oxidoreductase that catalyzes the irreversible reduction of testosterone to 5-alpha-dihydrotestosterone (DHT), a more potent androgen crucial for prostate development and male sexual differentiation. Related references: PMID:10898110, PMID:23685396
• It can also act on other 3-oxo steroids such as progesterone, contributing to broader steroid hormone metabolism.
• The enzyme is localized to the membrane of the endoplasmic reticulum and microsomes, consistent with its role in lipid/steroid metabolism.
• SRD5A2 is expressed at high levels in the prostate and other androgen-sensitive tissues, underscoring its importance in androgen signaling.
• The protein contains multiple transmembrane helices and is anchored in intracellular membranes, facilitating its contact with lipophilic substrates.
• Mutations in SRD5A2 are associated with 46,XY disorder of sex development (pseudohermaphroditism), highlighting its critical role in human sexual differentiation.
• UniProt annotates this protein with keywords including Differentiation, Disease variant, Lipid metabolism, and NADP, reflecting its biological significance and clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide covering amino acids 1-100 of human SRD5A2, suggesting the antibody likely recognizes an epitope in the N-terminal region.
• For Western blot, consider using reducing conditions; an expected band around 28 kDa should be detected in androgen-sensitive cell lines such as LNCaP or PC-3.
• In ELISA applications, direct or capture formats may be optimized; users should titrate the antibody and validate with appropriate controls.
• Cross-reactivity with mouse and rat allows for translational studies, but species-specific expression patterns should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibodies, bulk reagents, and tailored procurement solutions for challenging targets. This anti-SRD5A2 monoclonal antibody supports research in androgen metabolism and prostate disease. Our supply capabilities include antibody pairs, optimized monoclonals/polyclonals, and rare-target sourcing to streamline your diagnostic development.