Anti-SRD5A1 Polyclonal Antibody for WB, ELISA - P18405

Core Product Specifications and Parameters

Biological Background: SRD5A1 Function and Localization

• Catalyzes the irreversible stereospecific reduction of the delta 4,5 double bond of various 3-oxo steroids, producing their 5alpha-dihydro-3-oxo forms in an NADPH-dependent manner. Related references: PMID:2339109 PMID:23685396
• Converts testosterone into the more potent androgen 5-alpha-dihydrotestosterone (DHT), the preferred ligand for androgen receptor transactivation, playing a key role in male sexual differentiation. Related references: PMID:2339109 PMID:23685396
• Also metabolizes other steroids, including progesterone, androstenedione, and corticoids, yielding metabolites with diverse biological activities. Related references: PMID:2339109
• Central to androgen physiology and sexual differentiation. Related references: PMID:2339109
• Localized to the microsome membrane and endoplasmic reticulum membrane.
• Expressed in liver and at a low level in prostate.
• Contains transmembrane helices and is annotated with keywords including NADP, oxidoreductase, lipid metabolism, and sexual differentiation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide (aa 100-200); the resulting polyclonal antibody may preferentially detect denatured SRD5A1 in WB. Consider optimizing denaturing conditions for your samples.
• Validated applications are WB and ELISA. For ELISA, explore its utility as a capture or detection antibody in your specific assay setup.
• Cross-reactivity is reported for mouse and rat; validate endogenous detection in these species if needed.
• Always titrate the antibody in your experimental system to determine the optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs by offering a streamlined sourcing channel for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-SRD5A1 product, and bulk ancillary reagents. Whether your work focuses on androgen signaling, steroid metabolism, or sexual differentiation, CamelBio can assist in securing specialized reagents and rare-target raw materials to bridge the gap from concept to clinic.