Anti-Src Rabbit Polyclonal Antibody for WB and ELISA - P12931

Core Product Specifications and Parameters

Biological Background: Src Function and Localization

• Receptor-mediated activation: Activated by engagement of various cellular receptors, including immune and integrin receptors, initiating signaling cascades that control gene transcription, apoptosis, and migration (PubMed:34234773). Related references: PMID:34234773
• Cell adhesion and cytoskeleton: Phosphorylates focal adhesion proteins PTK2/FAK1 and paxillin, linking integrin signaling to actin cytoskeleton reorganization and cell-matrix adhesion (PubMed:21411625). Related references: PMID:21411625
• Osteoclast function: Essential for bone resorption; forms a complex with PTK2B/PYK2 and phosphorylates CBL, leading to PI3K recruitment and osteoclast activation (PubMed:14585963, PubMed:8755529). Related references: PMID:14585963 PMID:8755529
• Apoptosis regulation: Phosphorylates caspase-8 at Tyr-380, suppressing its processing and proapoptotic function (PubMed:16619028). Related references: PMID:16619028
• Subcellular localization and expression: Localizes to the plasma membrane, mitochondrial inner membrane, nucleus, and focal adhesions. Ubiquitously expressed, with highest levels in platelets, neurons, and osteoclasts.
• Oncogenic potential: Functions as a proto-oncogene; enables anchorage-independent cell growth and is frequently implicated in cancer (PubMed:19307596). Related references: PMID:19307596

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 410–536 of human Src, covering the C-terminal portion of the kinase domain. Validate that the antibody recognizes your target isoform or phosphorylation state in the intended application.
• For Western blotting, expect a band near 60 kDa. Use appropriate positive control lysates (e.g., A431, HeLa) and optimize blocking and antibody concentration for your samples.
• For ELISA, perform checkerboard titration to determine optimal coating antigen and antibody dilution. Use an HRP-conjugated anti-rabbit secondary antibody for detection.
• If applied to other techniques such as immunoprecipitation or immunohistochemistry, confirm performance independently, as these are not listed in the product specifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For Src kinase signaling studies, we offer validated antibodies, recombinant proteins, and ancillary reagents, as well as custom sourcing for rare targets. Let CamelBio streamline your assay development with reliable, scalable supply solutions.