Anti-SRA1 Polyclonal Antibody for WB, ELISA - Q9HD15

Core Product Specifications and Parameters

Biological Background: SRA1 Function and Localization

• SRA1 (Steroid receptor RNA activator 1), also known as SRAP, is a gene that produces both a functional non-coding RNA and a protein coactivator involved in transcriptional regulation.
• The protein form of SRA1 acts as a coactivator for steroid receptors, enhancing both ligand-independent (through AF-1 domain) and ligand-dependent (through AF-2 domain) transactivation.
• It plays roles in promoting cellular proliferation and differentiation and can induce apoptosis, linking it to developmental processes and tumorigenesis.
• SRA1 localizes to both the nucleus and cytoplasm, facilitating its involvement in multiple cellular pathways.
• It is highly expressed in liver, skeletal muscle, and brain, with significant upregulation observed in breast, ovarian, and uterine tumors, underscoring its clinical relevance.
• Post-translational modifications include phosphorylation, and SRA1 may function as part of ribonucleoprotein complexes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 1–100 of human SRA1 (NP_001030312.2); ensure target protein integrity in this region when planning experiments.
• For Western blotting, a band around 24 kDa is expected; human liver or skeletal muscle lysates may serve as positive controls due to high SRA1 expression.
• When performing ELISA, consider using the recombinant SRA1 protein or the immunogenic peptide for standard curve generation and assay validation.
• This antibody cross-reacts with human and rat; for other species, experimental verification is recommended.
• Optimal antibody dilution should be determined empirically for each assay and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials for researchers and diagnostic developers, including this high-quality anti-SRA1 polyclonal antibody for transcriptional regulation and cancer studies. Beyond individual antibodies, we provide validated antibody pairs, bulk ancillary reagents, and assist with rare target raw-material sourcing to accelerate your assay development. Whatever your project’s scale, CamelBio is your trusted partner from concept to clinic.