Anti-SR Protein Repeat Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q13247

Core Product Specifications and Parameters

Biological Background: SR Protein Repeat (SRSF6/SRP55) Function and Localization

• Also known as Pre-mRNA-splicing factor SRP55, Splicing factor arginine/serine-rich 6 (SRSF6), and B52.
• Functions in constitutive pre-mRNA splicing and regulates alternative splice site selection.
• Modulates alternative splicing of MAPT/Tau exon 10, thereby influencing tau protein isoform expression.
• Binds to alternative exons of tenascin-C (TNC) pre-mRNA and promotes the expression of alternatively spliced TNC variants.
• Involved in wound healing and keratinocyte differentiation/proliferation through its role in alternative splicing regulation.
• Localizes to the nucleus and accumulates in nuclear speckles, which are sites of active splicing and mRNA processing.
• Post-translational modifications include phosphorylation, acetylation, and isopeptide bond formation (ubiquitination), which may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 of human SRSF6; high sequence conservation with mouse and rat supports the observed cross-reactivity.
• For western blot, a band of approximately 40 kDa is expected; use appropriate nuclear loading controls and validate in relevant cell lines or tissues.
• IHC-P may require antigen retrieval; confirm staining patterns in tissues known to express SRSF6, such as those with active splicing.
• IP and ELISA can be optimized by titrating antibody concentration and using appropriate positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This SR protein repeat antibody supports splicing-related research and assay development, and our sourcing platform can assist with rare target procurement and technical consultation to meet your specific program needs.