Anti-SR-B2/LIMPII Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q14108

Core Product Specifications and Parameters

Biological Background: SR-B2/LIMPII Function and Localization

• SR-B2/LIMPII, also known as lysosome membrane protein 2 (LIMP‑2), LGP85, CD36L2, SCARB2, is a multi‑pass membrane glycoprotein primarily localized to the lysosomal membrane.
• It functions as a specific lysosomal receptor for glucosylceramidase (GBA1), mediating its transport and targeting to lysosomes; defects in this interaction are linked to lysosomal dysfunction.
• The protein belongs to the scavenger receptor class B family (CD36 family) and shares structural features such as a large lumenal domain, short transmembrane segments, and a cytoplasmic tail.
• Alternative splicing generates multiple isoforms; the canonical isoform has a calculated molecular weight of approximately 54 kDa, consistent with the SWISS annotation.
• SR-B2/LIMPII contains several post‑translational modifications, including N‑glycosylation and disulfide bonds, which are important for protein folding and stability.
• In addition to its endogenous function, the protein acts as a host cell receptor for enterovirus 71, facilitating viral entry and infection.
• Keywords associated with this protein in UniProt include epilepsy, neurodegeneration, and disease variant, reflecting its association with several neurological disorders.
• It is widely expressed, and its lysosomal membrane residency makes it a useful marker for lysosomal compartments in cell biology studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1‑100 of human SR-B2/LIMPII; this N‑terminal region is predicted to be exposed on the lumenal side of the lysosomal membrane.
• For western blot (WB), expect a band around 54 kDa; note that glycosylation may cause a slight shift or diffuse appearance.
• In immunohistochemistry (IHC‑P), validate the antibody in appropriate positive control tissues known to express SR-B2/LIMPII, using antigen retrieval as needed.
• This antibody is reported to cross‑react with mouse and rat; when using these species, consider confirming reactivity in the exact sample type of interest.
• For ELISA applications, titrate the antibody appropriately and use a suitable blocking agent to minimize non‑specific binding.
• Since SR-B2/LIMPII is a membrane protein, use gentle lysis conditions that preserve membrane integrity for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing IVD manufacturers, reference labs, and research institutes with a streamlined one‑stop access to high‑quality raw materials, from concept to clinical application. Our range includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, as well as bulk ancillary reagents, designed to accelerate diagnostic development. If your project requires rare‑target raw materials for lysosomal biology, viral entry mechanisms, or neurodegenerative disease research, we offer flexible sourcing and supply solutions to meet your technical and scale‑up needs.