Anti-SR-B2/LIMPII Polyclonal Antibody for WB, IF/ICC, ELISA - Q14108

Core Product Specifications and Parameters

Biological Background: SR-B2/LIMPII Function and Localization

• SR-B2/LIMPII (also known as lysosome membrane protein 2, LGP85, CD36L2) is a multi-ligand receptor of the CD36 family, primarily localized to the lysosomal membrane.
• It acts as the main lysosomal receptor for glucosylceramidase (GBA1), mediating its transport from the endoplasmic reticulum to lysosomes.
• Proper GBA1 targeting is critical for glycosphingolipid degradation; SCARB2 mutations cause action myoclonus-renal failure syndrome and can affect glucocerebrosidase activity, linking it to Gaucher disease and neurodegeneration.
• As a host cell receptor, SR-B2/LIMPII facilitates cellular entry of enterovirus 71, contributing to viral pathogenesis.
• The protein spans the lysosomal membrane with a large luminal domain and a short cytoplasmic tail; the luminal domain interacts with GBA1 and viruses.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are essential for folding and function.
• Alternative splicing generates multiple isoforms, potentially diversifying its functions in different tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200-380 within the luminal domain, so the antibody may detect both fully processed and unglycosylated forms of the protein.
• For Western blot, consider standard denaturing conditions and validate in human, mouse, or rat lysates based on reported cross-reactivity; the predicted band is around 54 kDa, but glycosylation may shift migration.
• In immunofluorescence/immunocytochemistry, permeabilization is required to access the intralysosomal epitope; including lysosomal markers as co-stains is advised.
• For ELISA, test direct coating of recombinant protein or capture antibody formats; optimize concentrations with the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop procurement partner for IVD raw materials, offering a comprehensive portfolio of antibodies and reagents. This anti-SR-B2/LIMPII antibody supports investigations into lysosomal biology and viral entry mechanisms. We provide validated antibody pairs, optimized bulk polyclonal/monoclonal antibodies, and ancillary reagents to streamline diagnostic development. Our sourcing expertise extends to rare targets and custom formulations, helping you bridge the gap from concept to clinic efficiently.