Anti-SQSTM1/p62 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q13501

Core Product Specifications and Parameters

Biological Background: SQSTM1/p62 Function and Localization

• SQSTM1/p62 (Sequestosome-1) is a ubiquitin-binding scaffold protein encoded by the SQSTM1 gene in humans, also referred to as EBIAP, p60, p62, and ZIP3 (UniProt Q13501).
• It functions as a selective autophagy receptor, bridging polyubiquitinated proteins to autophagosomes for lysosomal degradation. Related references: PMID:15340068 | PMID:15953362 | PMID:16286508
• Upon binding ubiquitinated cargo via its UBA domain, SQSTM1 undergoes liquid-liquid phase separation to form cytoplasmic p62 bodies; its LIR motif subsequently recruits ATG8 family proteins, leading to cargo and SQSTM1 clearance. Related references: PMID:15911346 | PMID:20168092 | PMID:22017874 | PMID:16286508 | PMID:17580304
• SQSTM1 activates the NFE2L2/NRF2 antioxidant pathway by sequestering KEAP1 into inclusions, thereby promoting NRF2 nuclear translocation and cytoprotective gene expression. Related references: PMID:20452972 | PMID:28380357 | PMID:33393215
• Beyond aggrephagy, SQSTM1 facilitates pexophagy by targeting ubiquitinated PEX5 to autophagosomes, aids STING1 trafficking, and organizes the endosomal pathway via vesicle retention. Related references: PMID:26344566 | PMID:29496741 | PMID:27368102 | PMID:33472082
• It additionally promotes proteasomal degradation of tensin TNS2 and regulates NFKB1 activation by cytokines such as TNF, NGF, and IL-1. Related references: PMID:25101860 | PMID:10356400 | PMID:10747026 | PMID:11244088
• Subcellularly, SQSTM1 localizes to autophagosomes, preautophagosomal structures, cytosol, nucleus (including PML bodies), late endosomes, lysosomes, and the endoplasmic reticulum.
• Clinically, SQSTM1 mutations are linked to amyotrophic lateral sclerosis (ALS), frontotemporal dementia, and Paget disease of bone, emphasizing its role in neurodegeneration and bone homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-356) of human SQSTM1/p62; the antibody may detect full-length (~48 kDa) and N-terminal fragments in WB.
• For IF/ICC, expect punctate cytoplasmic staining (p62 bodies); co-staining with ubiquitin or LC3 is recommended to confirm autophagy-related localization.
• For IP, consider using lysis buffers with detergents to solubilize aggregated SQSTM1; optimization may be needed for your sample type.
• This antibody shows cross-reactivity with mouse and rat; validate species-specific performance in your experimental context before use.
• As a polyclonal antibody, we recommend verifying lot-to-lot consistency for quantitative applications such as ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to diagnostics manufacturers and research institutes. This anti-SQSTM1/p62 antibody is a powerful tool for autophagy, ubiquitin-proteasome, and neurodegeneration research, and we support your entire workflow from concept to clinic. Contact us today for custom sourcing solutions and dedicated technical assistance.