Anti-SQSTM1/p62 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13501

Core Product Specifications and Parameters

Biological Background: SQSTM1/p62 Function and Localization

• SQSTM1/p62 (Sequestosome-1), also known as p62, EBIAP, p60, and ubiquitin-binding protein p62, is a multifunctional scaffold protein involved in selective autophagy, signaling, and cellular stress responses.
• Core function as an autophagy receptor: SQSTM1 bridges polyubiquitinated proteins and autophagosomes. It undergoes liquid-liquid phase separation upon binding ubiquitinated cargo via its UBA domain, forming p62 bodies, and then engages ATG8 family proteins on autophagosomal membranes through its LIR motif, leading to degradation of both cargo and SQSTM1 itself. Related references: PMID:15340068 PMID:15953362 PMID:16286508 PMID:17580304 PMID:20168092 PMID:22017874 PMID:15911346 PMID:22622177 PMID:37802024
• Activates the NFE2L2/NRF2 antioxidant pathway by interacting with KEAP1, resulting in KEAP1 sequestration into inclusion bodies and NRF2 nuclear accumulation, triggering expression of cytoprotective genes. Related references: PMID:20452972 PMID:28380357 PMID:33393215
• Regulates endosomal organization: ubiquitinated by RNF26, SQSTM1 attracts specific vesicle-associated adapters, retaining vesicles in the perinuclear region to facilitate efficient endosomal transport. Related references: PMID:27368102 PMID:33472082
• Mediates pexophagy (selective autophagy of peroxisomes) by linking ubiquitinated PEX5 receptor to autophagosomes in response to oxidative stress. Related references: PMID:26344566
• Involved in innate immunity by promoting relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Related references: PMID:29496741
• Participates in NF-kappa-B signaling regulation downstream of TNF, NGF, and interleukin-1, and acts as an adapter between TRAF6 and CYLD. Related references: PMID:10356400 PMID:10747026 PMID:11244088
• Ubiquitously expressed across tissues and localizes to diverse subcellular compartments including autophagosomes, late endosomes, lysosomes, nucleus, endoplasmic reticulum, and cytoplasmic p62 bodies.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-440 of human SQSTM1 (NP_003891.1). This region includes the PB1, ZZ-type zinc finger, and ubiquitin-associated (UBA) domains, and may recognize full-length endogenous SQSTM1/p62.
• For Western blot (WB), expect a band at approximately 48 kDa. SQSTM1 is degraded by autophagy; treatment with lysosomal inhibitors (e.g., chloroquine or bafilomycin A1) can increase detection levels.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval conditions and validate with appropriate controls, as SQSTM1 forms distinct cytoplasmic puncta (p62 bodies) that are enriched under stress conditions.
• Cross-reactivity is reported for human, mouse, and rat; however, it is advisable to verify performance in your specific sample system if using non-human species.
• For ELISA, the antibody may be employed as a capture or detection reagent after careful optimization of coating and detection conditions.

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