Anti-SQSTM1/p62 Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q13501

Core Product Specifications and Parameters

Biological Background: SQSTM1/p62 Function and Localization

• SQSTM1/p62 (Sequestosome-1) is a multidomain scaffold protein that functions as a selective autophagy adapter, linking ubiquitinated cargo to the autophagic machinery. Related references: PMID:15340068, PMID:15953362, PMID:16286508

• It promotes formation of p62 bodies via liquid-liquid phase separation upon binding polyubiquitinated proteins through its C-terminal UBA domain, and recruits ATG8 family proteins via its LIR motif to deliver cargo to autophagosomes. Related references: PMID:15911346, PMID:20168092, PMID:22017874, PMID:17580304

• SQSTM1 activates the NFE2L2/NRF2 antioxidant pathway by sequestering KEAP1 into inclusion bodies, leading to nuclear accumulation of NRF2 and expression of cytoprotective genes. Related references: PMID:20452972, PMID:28380357, PMID:33393215

• It participates in pexophagy by bridging ubiquitinated PEX5 receptors to autophagosomes under oxidative stress. Related references: PMID:26344566

• In endosome organization, SQSTM1 is ubiquitinated by RNF26, which attracts vesicle adapters and retains vesicles in the perinuclear cloud for efficient cargo transport. Related references: PMID:27368102, PMID:33472082

• It may regulate NF-κB activation in response to TNF-α, NGF, and IL-1. Related references: PMID:10356400, PMID:10747026, PMID:11244088

• The protein localizes to autophagosomes, cytoplasmic vesicles, PML nuclear bodies, late endosomes, lysosomes, endoplasmic reticulum, and sarcomeres, reflecting its diverse cellular roles.

• It is ubiquitously expressed and implicated in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia, with mutations linked to impaired autophagy.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 341–440 of human SQSTM1/p62, covering a region within the functional UBA domain; this may confer cross-reactivity to orthologs and ensure detection of total protein.
• For western blotting, a band at approximately 48 kDa is expected; consider including positive and negative lysates to verify specificity.
• In immunofluorescence, p62 bodies appear as cytoplasmic puncta; fixation and permeabilization protocols should be optimized for the cell type.
• The antibody’s reported broad application range (ELISA, IP, IHC-P) suggests flexibility; dilutions and incubation conditions should be empirically validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner, supplying validated antibodies, bulk ancillary reagents, and rare target sourcing for diagnostic and research communities. This SQSTM1/p62-targeting monoclonal antibody exemplifies our commitment to high-quality tools for autophagy, neurodegeneration, and cell signaling studies. With reliable supply chains and technical support, CamelBio accelerates your path from concept to clinic.