Anti-SQS/FDFT1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P37268

Core Product Specifications and Parameters

Biological Background: SQS/FDFT1 (Squalene Synthase) Function and Localization

• Squalene synthase (SQS), encoded by the FDFT1 gene, is also known as farnesyl-diphosphate farnesyltransferase 1. It catalyzes the first committed step in sterol biosynthesis, converting two molecules of farnesyl pyrophosphate (FPP) into squalene.
• The enzymatic reaction proceeds in two distinct steps: first, the condensation of two FPP molecules to form presqualene diphosphate (PSQPP); second, the heterolysis, isomerization, and NADPH/NADH-dependent reduction of PSQPP to produce squalene.
• SQS is an integral membrane protein of the endoplasmic reticulum, with a single transmembrane helix anchoring it to the ER membrane.
• The enzyme is widely expressed across human tissues, consistent with the ubiquitous requirement for cholesterol synthesis.
• As a multifunctional enzyme, SQS requires divalent metal ions such as magnesium for catalytic activity and utilizes NAD(P)H as a cofactor.
• The FDFT1 gene undergoes alternative splicing, generating multiple transcript variants that may have distinct regulatory roles.
• SQS is a key enzyme in the cholesterol biosynthesis pathway and is implicated in lipid and steroid metabolism; it is a target for cholesterol-lowering therapies.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of human SQS is approximately 48 kDa; using appropriate positive control lysates (e.g., HepG2, HeLa) is recommended.
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., citrate buffer, pH 6.0) may be required for optimal detection in formalin-fixed paraffin-embedded tissues.
• For immunofluorescence/ICC, permeabilization with Triton X-100 or similar detergent can help access the endoplasmic reticulum-localized target.
• The antibody cross-reacts with mouse and rat, allowing its use in rodent models for cholesterol metabolism research.
• As a polyclonal antibody, batch-to-batch consistency should be validated by the end-user, especially for quantitative assays like ELISA.
• Consider using this antibody in combination with other markers to study ER localization or cholesterol synthesis pathway interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing solution for diagnostic manufacturers and research laboratories, offering this anti-SQS/FDFT1 rabbit polyclonal antibody along with a comprehensive portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents for cholesterol metabolism studies. Our one-stop platform enables efficient procurement of optimized monoclonal and polyclonal antibodies, bulk raw materials, and custom sourcing for rare or challenging targets, supporting your assay development from concept to clinic.