Anti-SQS/FDFT1 Rabbit Monoclonal Antibody for WB, ELISA - P37268

Core Product Specifications and Parameters

Biological Background: SQS/FDFT1 Function and Localization

• Squalene synthase (SQS), encoded by the FDFT1 gene, is a dual-function enzyme that catalyzes the first committed step of sterol biosynthesis, producing squalene from two farnesyl pyrophosphate (FPP) molecules.
• The reaction proceeds in two distinct half-reactions: initially forming presqualene diphosphate (PSQPP) from two FPP, then heterolysis, isomerization, and NADPH/NADH-dependent reduction to squalene.
• As the first committed enzyme in the cholesterol biosynthetic pathway, SQS plays a critical role in regulating intracellular cholesterol levels and is a key control point for sterol homeostasis.
• Alternative splicing of FDFT1 produces multiple isoforms; the canonical isoform localizes to the endoplasmic reticulum membrane via a C-terminal transmembrane helix.
• Widely expressed across human tissues, SQS activity is particularly significant in liver, intestine, and steroidogenic organs where high-rate cholesterol synthesis occurs.
• Post-translational modifications and structural features include magnesium-dependent activity, NAD(P) cofactor binding, and a catalytic mechanism involving multiple metal-binding sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal peptide (aa 150-250) of human SQS/FDFT1, suggesting the antibody may recognize epitopes conserved across species; confirm specificity in the relevant experimental model.
• For Western blotting, the expected molecular weight is approximately 48 kDa; consider using appropriate positive controls (e.g., liver or HeLa cell lysates) and validate band identity with knockdown or knockout samples.
• In ELISA applications, optimizing antibody concentration and blocking conditions is recommended to minimize background; cross-reactivity with mouse and rat SQS can facilitate translational studies but should be verified independently.
• Reducing and non-reducing SDS-PAGE may impact detection; standardize gel conditions and membrane transfer protocols for consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research teams with high-quality IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For cholesterol metabolism and lipid biosynthesis research, we provide reliable anti-SQS/FDFT1 reagents and can assist in sourcing additional targets such as HMGCR or LDLR. With a commitment to streamlined procurement and technical support, CamelBio accelerates your journey from discovery to diagnostic application.