Anti-SQLE Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14534

Core Product Specifications and Parameters

Biological Background: SQLE Function and Localization

• SQLE encodes squalene monooxygenase (also known as squalene epoxidase, SE), a key enzyme in the cholesterol biosynthesis pathway.
• It catalyzes the stereospecific conversion of squalene to (S)-2,3-epoxysqualene, representing the first oxygenation step and a rate-limiting reaction in sterol biosynthesis.
• The enzyme localizes to the endoplasmic reticulum and microsomal membranes, consistent with its role in lipid metabolism.
• SQLE is predominantly expressed in the liver, the primary site of cholesterol synthesis and homeostasis.
• As a flavoprotein, SQLE requires FAD as a cofactor for its oxidoreductase activity.
• Post-translational modifications include ubiquitin conjugation (Ubl conjugation) as indicated by proteomics studies.
• The enzyme is part of the reference proteome, with its 3D structure solved, aiding in understanding its catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen is designed against amino acids 300–400 of human SQLE; consider epitope accessibility and validate specificity in the relevant species (human, mouse, rat).
• For Western blotting, the predicted molecular weight is ~64 kDa; include appropriate positive controls (e.g., liver lysate) and confirm band specificity.
• In immunohistochemistry (IHC-P), optimization of antigen retrieval and detection conditions is recommended; validate staining in target tissues such as liver.
• For immunofluorescence (IF/ICC), co-staining with ER markers can help confirm subcellular localization.
• ELISA performance may depend on coating conditions; consider using recombinant SQLE protein to establish assay parameters.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-SQLE polyclonal antibody for cholesterol metabolism studies. From monoclonal and polyclonal antibodies to recombinant proteins and ancillary reagents, we streamline sourcing for every stage of assay development. Our technical team can also assist in sourcing rare or custom targets to meet specific project needs.