Anti-SPZ1 Polyclonal Antibody for WB and ELISA - Q9BXG8

Core Product Specifications and Parameters

Biological Background: SPZ1 Function and Localization

• SPZ1 (Spermatogenic leucine zipper protein 1), also known as testis-specific protein 1 (NYD-TSP1), is a transcription factor that binds to DNA E-box (5'-CANNTG-3') and G-box motifs.
• It contains a coiled-coil domain and is classified as a phosphoprotein, suggesting regulation by post-translational modifications.
• SPZ1 localizes to both the cytoplasm and nucleus, consistent with its role in transcriptional regulation.
• It is specifically and strongly expressed in the testis, implying a critical function in spermatogenesis.
• SPZ1 may regulate cell proliferation and differentiation during spermatogenesis, as suggested by its DNA-binding activity.
• Expression has also been detected in several tumor cell lines, indicating potential roles in oncogenesis.
• The protein has a molecular weight of approximately 49.4 kDa.
• Keywords from UniProt include: Coiled coil, DNA-binding, Nucleus, Cytoplasm, Phosphoprotein, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen covers nearly the full-length human SPZ1 protein (aa 1-430), making this antibody well-suited for detecting endogenous SPZ1 in human and rat testis lysates or cell line extracts by WB.
• For WB, optimize the primary antibody dilution using known positive controls such as testis tissue or tumor cell lines. Non-specific bands may appear; confirm specificity by comparing with negative controls.
• In ELISA, validate binding with the recombinant immunogen or native protein from appropriate samples. Pair with a suitable secondary antibody and optimize coating conditions.
• The antibody cross-reacts with rat SPZ1; for other species, sequence homology should be checked before use.
• As a polyclonal antibody, slight lot-to-lot variation may occur. Always perform a pilot experiment when switching to a new batch.
• Store according to the shipping and handling instructions; repeated freeze-thaw cycles should be avoided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For SPZ1-related studies, we supply validated polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Our global supply network ensures rapid delivery and reliable support for your reproductive biology and oncology assay development.