Anti-SPRY3 Rabbit Polyclonal Antibody for WB, ELISA - O43610

Core Product Specifications and Parameters

Biological Background: SPRY3 (Protein sprouty homolog 3) Function and Localization

• SPRY3 encodes Protein sprouty homolog 3, also known as Sprouty RTK signaling antagonist 3 or Sprouty3.
• It negatively regulates the MAPK cascade, resulting in reduced extracellular matrix protein accumulation. Related references: PMID:30878395
• May act as an antagonist of fibroblast growth factor (FGF) pathways and negatively modulate respiratory organogenesis. Related references: PMID:9458049
• Inhibits neurite branching, arbor length, and neurite complexity, and inhibits EGF-mediated p42/44 ERK signaling (by similarity).
• Localized predominantly in the cytoplasm.
• Widely expressed, with highest expression in fetal tissues; in adult, prominently expressed in brain (Purkinje cells of cerebellum) and heart myocardium. Related references: PMID:26089202, PMID:30878395

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide, enabling detection of full-length SPRY3 and potential isoforms.
• For Western blotting, the predicted molecular weight is ~31 kDa; select appropriate gel percentage and transfer conditions.
• In ELISA, validate specificity using recombinant SPRY3 protein or relevant cell lysates.
• When analyzing brain or heart tissue samples, consider enrichment or use positive controls such as cerebellum lysate due to high Purkinje cell expression.
• Cross-reactivity is confirmed for human; for other species, verify sequence homology and perform pilot experiments.

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CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including this anti-SPRY3 polyclonal antibody for MAPK signaling and neurogenesis studies. Our portfolio covers validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to streamline your assay development. For rare or custom targets, our sourcing team leverages global networks to deliver reliable materials from concept to clinic.