Anti-SPRR3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UBC9

Core Product Specifications and Parameters

Biological Background: SPRR3 Function and Localization

• SPRR3 (Small proline-rich protein 3) is also designated as 22 kDa pancornulin, cornifin beta, or esophagin, reflecting its expression in different epithelial tissues.
• It functions as a cross-linked envelope protein of keratinocytes, playing a structural role in the formation of the cornified cell envelope, a critical barrier against environmental insults.
• Subcellularly, SPRR3 is localized to the cytoplasm, where it participates in the assembly of the cornified envelope during terminal differentiation.
• The protein features acetylated residues and contains repetitive amino acid sequences, which contribute to its structural properties.
• Direct protein sequencing has confirmed the expression of SPRR3 at the protein level, supporting its classification as a reference proteome component.
• The predicted molecular weight of SPRR3 is ~18 kilodaltons (18,154 Da); however, the observed molecular weight by SDS-PAGE is approximately 17 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 1–70 of human SPRR3, a region well conserved in mouse and rat orthologs—consistent with the demonstrated cross-reactivity. Verify species-specific reactivity if using beyond these species.
• For Western blot, use keratinocyte-rich lysates (e.g., skin, esophagus) or SPRR3-expressing cell lines as positive controls, and expect a band at ~17 kDa. Optimize blocking and antibody dilution for your specific system.
• In immunofluorescence/ICC, target the cytoplasmic localization of SPRR3; standard fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) are usually suitable. Include appropriate negative controls.
• For ELISA applications, the antibody can be used for direct or capture detection of SPRR3; however, pairing with a validated detection antibody may enhance assay sensitivity.
• As a polyclonal antibody, lot-to-lot consistency should be verified in quantitative assays; consider internal reference standards to normalize results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. The SPRR3 polyclonal antibody highlights our commitment to supporting keratinocyte biology and dermatology-focused research by supplying high-quality reagents for cornified envelope studies. From concept to clinic, CamelBio provides integrated technical and sourcing consultation to streamline assay development workflows.