Anti-SPRR1A Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P35321

Core Product Specifications and Parameters

Biological Background: SPRR1A (Cornifin-A) Function and Localization

• SPRR1A encodes Cornifin-A, a 19 kDa pancornulin (also known as SPRK or SPR-IA), a member of the small proline-rich protein family pivotal for keratinocyte differentiation.
• It functions as a cross-linked envelope protein of keratinocytes, contributing to the assembly of an insoluble cornified envelope beneath the plasma membrane.
• Cornifin-A is initially cytosolic but becomes cross-linked to membrane proteins by transglutaminase, a key enzymatic step in epidermal barrier formation.
• Subcellular localization is primarily cytoplasmic, consistent with its precursor role before translocation and cross-linking at the cell periphery.
• The protein plays a critical role in keratinization, providing structural integrity and mechanical resistance to the epidermis.
• It contains repetitive proline-rich motifs, a characteristic feature of the SPRR family, which facilitate its structural flexibility and cross-linking capability.
• Additional annotations include direct protein sequencing and proteomics identification, confirming its presence in the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen, a synthetic peptide spanning amino acids 1‑89 of human SPRR1A, is designed to target the N‑terminal region; consider sequence alignment to verify epitope conservation across species.
• For Western blot (WB), a band near 10 kDa is expected; validate with appropriate positive controls such as keratinocyte lysates or skin tissue extracts.
• In immunofluorescence (IF‑P), nuclear/perinuclear staining may be observed in differentiating keratinocytes; optimize permeabilization and fixation protocols for your cell model.
• ELISA can be used for peptide or protein quantification; pairing with a matched detection antibody may improve sensitivity.
• Cross‑reactivity with mouse and rat is reported; confirm reactivity in your specific sample type and application using appropriate control tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers, laboratories, and research institutes, offering end‑to‑end support from concept to clinic. This SPRR1A polyclonal antibody supports dermatology and skin barrier research, with access to validated antibodies, optimized monoclonal/polyclonal reagents, and bulk ancillary raw materials. Leverage CamelBio’s network to source rare targets and scale your immunoassay development efficiently.