Anti-Sprouty 2 Monoclonal Antibody for WB, IHC-P, ELISA - O43597

Core Product Specifications and Parameters

Biological Background: Sprouty 2 Function and Localization

• Encodes protein sprouty homolog 2 (SPRY2), a member of the sprouty family, also designated IGAN3 or hSPRY2.
• Acts as an antagonist of fibroblast growth factor (FGF) signaling by inhibiting FGF-mediated phosphorylation of ERK1/2, thereby negatively regulating cell proliferation and differentiation (By similarity).
• Suppresses TGFB-induced epithelial-to-mesenchymal transition in retinal lens epithelial cells, suggesting a role in maintaining epithelial phenotype (By similarity).
• Interferes with CBL/C-CBL-mediated ubiquitination of the epidermal growth factor receptor (EGFR), prolonging EGFR signaling (PubMed:17974561). Related references: PMID:17974561
• Localizes to the cytoplasm and cytoskeleton, and is enriched at cell membrane ruffles and projections, consistent with its role in receptor tyrosine kinase signaling.
• Involved in developmental processes including retinal lens fiber differentiation, limb bud outgrowth, and respiratory organogenesis (By similarity).
• Belongs to the reference proteome and features a known 3D structure, aiding structural and functional studies.

Experimental Guidance and Technical Tips

• For Western blot analysis, a signal near 35 kDa is anticipated for human Sprouty 2; validate band specificity in your sample system.
• For immunohistochemistry (IHC-P), consider standard antigen retrieval protocols and confirm staining patterns in human and rat tissue sections.
• The immunogen targets a sequence within amino acids 216–315, a region that may be conserved; verify cross-reactivity with rat lysates.
• For ELISA applications, optimize coating and detection conditions using recombinant Sprouty 2 or cell lysates.
• The antibody is a monoclonal reagent; lot-to-lot consistency can be expected, but validate performance in your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement pathway for high-quality IVD raw materials, including this anti-Sprouty 2 monoclonal antibody, serving diagnostic manufacturers, research laboratories, and CROs. With a portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, CamelBio supports the development of assays targeting FGF signaling, developmental pathways, and cancer research. For rare or custom targets like Sprouty 2, our sourcing network ensures flexible supply solutions from concept to clinical application.