Anti-SPR (Sepiapterin Reductase) Polyclonal Antibody for WB, ELISA - P35270

Core Product Specifications and Parameters

Biological Background: Sepiapterin Reductase (SPR) Function and Localization

• Sepiapterin reductase (SPR), also known as SDR38C1, is an NADP-dependent oxidoreductase involved in the final steps of tetrahydrobiopterin (BH4) biosynthesis.
• It catalyzes the reduction of 6-pyruvoyltetrahydropterin to 5,6,7,8-tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydroxylases and nitric oxide synthases.
• SPR is primarily localized in the cytoplasm, consistent with its role in BH4 production.
• Post-translational modifications include acetylation and phosphorylation; mutations in SPR are associated with dystonia (a disease variant).
• Keywords from UniProt highlight its involvement in oxidoreductase activity, NADP binding, and relevance to dystonia; a 3D structure is available.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human SPR protein (amino acids 1-261), so the antibody is expected to recognize endogenous SPR in human, mouse, and rat samples.
• In Western blot (WB), a band around 28 kDa should be detected; consider optimizing antibody dilution and sample preparation for your specific system.
• For ELISA, the polyclonal nature may provide robust signal; however, validate with purified SPR or appropriate cell lysates to establish sensitivity and specificity.
• When using for cross-species detection, confirm sequence homology between the target species to ensure reactivity.
• Store as recommended (shipped on ice bag) and avoid repeated freeze-thaw cycles to maintain antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to a broad portfolio of IVD raw materials, technical services, and consulting. This anti-SPR polyclonal antibody supports research into tetrahydrobiopterin metabolism and dystonia-related pathways. Through our global network, we source validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and even rare target raw materials—bridging your project from concept to clinic efficiently.