Anti-SPPL2A Polyclonal Antibody for WB, IHC-P, ELISA - Q8TCT8

Core Product Specifications and Parameters

Biological Background: SPPL2A Function and Localization

• SPPL2A is an intramembrane-cleaving aspartic protease (I-CLiP) that processes type II membrane signal peptides, including FASLG, ITM2B, and TNF. Related references: PMID:16829951, PMID:16829952, PMID:17557115

• It catalyzes the intramembrane cleavage of the anchored fragment of shed TNF, promoting the release of the intracellular domain (ICD) for signaling to the nucleus. Related references: PMID:16829952

• SPPL2A is responsible for the intramembrane cleavage of Fas antigen ligand FASLG, releasing the intracellular FasL domain (FasL ICD). Related references: PMID:17557115

• It is essential for degradation of the invariant chain CD74, which is central to antigen-presenting cell function in the immune system.

• The protease plays a role in regulating innate and adaptive immunity. Related references: PMID:16829952

• SPPL2A cleaves the simian foamy virus envelope glycoprotein gp130 independently of prior ectodomain shedding. Related references: PMID:23132852

• Localizes to the late endosome and lysosome membranes.

• Ubiquitously expressed in tissues; contains a signal peptide, multiple transmembrane helices, and glycosylation sites.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using whole-cell lysates from human, mouse, or rat cell lines or tissues; optimize antibody dilution and blocking conditions experimentally.
• For immunohistochemistry (IHC-P), validate antigen retrieval methods and include appropriate controls for your tissue of interest.
• For ELISA applications, the immunogen region (aa 421-520) may be suitable for capture or detection designs; pilot experiments with recombinant SPPL2A are recommended.
• As a polyclonal antibody, batch-to-batch variability may occur—always validate performance in the relevant sample system.
• SPPL2A is a membrane protein; efficient lysis and solubilization techniques may be required for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. This anti-SPPL2A polyclonal antibody is suited for researchers and diagnostic developers studying intramembrane proteolysis, immunology, and viral host interactions. Contact CamelBio to discuss your specific procurement needs and accelerate your project from concept to clinic.