Anti-SPOP Rabbit Monoclonal Antibody for WB, ELISA - O43791

Core Product Specifications and Parameters

Biological Background: SPOP Function and Localization

• Speckle-type POZ protein (SPOP), also known as HIB homolog 1 or Roadkill homolog 1, is the adaptor component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex.
• The complex mediates ubiquitination and typically proteasomal degradation of substrates including BRMS1, DAXX, PDX1/IPF1, GLI2, and GLI3. Homodimeric SPOP exhibits higher ligase activity than heterodimers with SPOPL.
• SPOP localizes to the nucleus, nuclear speckles, and cytoplasm.
• Widely expressed across tissues.
• Functions as a host antiviral factor: induces lysosome-dependent degradation of enterovirus EV71 protease 2A via 'Lys-48'-linked polyubiquitination. Related references: PMID:37796126
• Promotes ubiquitination and degradation of HNF1A to inhibit hepatitis B virus transcription and replication. Related references: PMID:38018242
• Regulates BET proteins BRD2, BRD3, and BRD4 stability. Related references: PMID:32109420
• Essential for proper translation of DNA replication licensing factors CDT1 and CDC6, contributing to DNA synthesis and cell proliferation. Related references: PMID:36791496
• Regulates IRF1 proteasomal turnover by targeting S/T-rich degrons. Related references: PMID:37622993
• Keywords: Host-virus interaction, intellectual disability, Ubl conjugation pathway; identified by mass spectrometry.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide (aa 250–350 of human SPOP), ensuring epitope specificity. For WB, expect a band at ~42 kDa. Validate in human-derived samples; cross-reactivity with other species is not tested.
• For ELISA, consider using the synthetic peptide as a positive control or coating antigen. Titration may be required to optimize signal-to-noise ratio.
• This antibody has not been validated for other applications (e.g., IHC, IP); performance should be determined empirically.
• Due to SPOP’s role in ubiquitination, consider protease inhibitor cocktails during sample preparation to preserve target integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, serving diagnostic manufacturers and research labs from concept to clinic. This anti-SPOP monoclonal antibody supports studies in ubiquitin-proteasome pathways, antiviral mechanisms, and cancer biology. Beyond this product, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, streamlining your assay development pipeline.